#SDS PAGE VS WESTERN BLOT VS ELISA VERIFICATION#
#SDS PAGE VS WESTERN BLOT VS ELISA ISO#

Introduction of Recombinant DNA Technology, Application of RDT, Recent trends and future perspective in RDT, Good laboratory practices (GLP), basic instrument handling, concept of basic calculation, Micropipette handling (forward and reverse pipetting techniques), Basics of competent cell, competent cell preparation, Introduction about ligation, T4 DNA Ligation of λ DNA by HindIII restriction enzyme, Basic concept of Agarose gel electrophoresis, Basic concept of Tank buffer (TBE/TAE) - 6X loading dye and ETBr solution, observe the efficiency of ligation reaction through Agarose gel electrophoresis, Overview of transformation, Transformation of ligated DNA, learn restriction-digestion technique (Plasmid DNA cut with EcoR1and HindIII), Perform Agarose gel electrophoresis of digested plasmid DNA, Basic concept of DNA fingerprinting by restriction fragment length polymorphism (RFLP) for bacterial strain identification and Blue-white screening. Genomics & Recombinant DNA Technology (Module 4) Introduction and scope of Cell and Molecular Biology, Overview of genomic DNA and RNA, General and safety rules for working in Lab, Demonstration of module oriented instruments, Basic concept of percentage, Molarity and normality, Principle-SOP and application of spectrophotometer, Types of extraction, Composition of buffers, Overview of distinguishing features of prokaryotic and eukaryotic cells, Overview of differential leukocyte cells, Preparation of blood smear and differential staining of blood cells, Genomic DNA/RNA isolation from plant, Genomic DNA/RNA isolation from Bacteria, Genomic DNA isolation from Hair/Nail/Blood, Basic concept for preparation of Tank buffer (TBE/TAE) - 6X loading dye and ETBr solution, Introduction and application of electrophoresis, Qualitative analysis of DNA/RNA through Agarose Gel Electrophoresis, Genomic DNA quantification & purity check through UV-VIS spectrophotometer, Basic concept of PCR, Performance of PCR in thermal cycler, Types of PCR (RT-PCR, Gradient PCR, Nested PCR and its application). if you apply for further studies or job (India or Abroad) in an from the date of certificate issued.

#SDS PAGE VS WESTERN BLOT VS ELISA VERIFICATION#

The verification of the certificate will be provided for life.

A Training Certificate (approved) will be issued with grades after successful completion of training program.

You can choose one module for a week, so that you can gain maximum knowledge in minimum durationĮxample: If you have duration of 1 week for training, you can opt any one module and get hands-on individual exposure on that and if you are coming for 30 days (4 weeks), so you can opt any 4 modules of your choice.

All the modules are industrially designed as per the requirements of Biotech sector.

NOIDA (Delhi - NCR), Mohali (PUNJAB),Ahmedabad, Dehradun,Hyderabad,Mumbai & Chennai.

#SDS PAGE VS WESTERN BLOT VS ELISA ISO#

is a leading ISO 9001:2015 certified company, with centers at

Rapture Biotech, India organizes Biotech summer/Winter training/Internship (short term) program each year to enhance hands on practical knowledge of life sciences students with latest Industry oriented courses.

Sds page vs western blot vs elisa